Description
Description: This assay employs a two-site sandwich ELISA to quantitate ZCCHC13 in samples. An antibody specific for ZCCHC13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ZCCHC13 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZCCHC13 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZCCHC13 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: ZCCHC13 appears to represent an intronless retrocopy of a related multi-exon gene located on chromosome 3. However, the CDS of this intronless gene remains relatively intact, it is conserved in other mammalian species, it is known to be transcribed, and it is therefore thought to encode a functional protein. The encoded protein contains six CCHC-type zinc fingers, and is thus thought to function as a transcription factor
This entry represents the CysCysHisCys (CCHC) type zinc finger domains, and have the sequence: C-X2-C-X4-H-X4-C
where X can be any amino acid, and number indicates the number of residues. These 18 residues CCHC zinc finger domains are mainly found in the nucleocapsid protein of retroviruses